Primer

Part:BBa_K4496013:Design

Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki   Group: iGEM22_USP-EEL-Brazil   (2022-09-20)


Primer to amplify psB1C3 (REV)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this primer, the pSB1C3 plasmid map was used. The point chosen for the design was ECORI. After the design, it was checked for hairpin.



Source

https://parts.igem.org/Part:pSB1C3

References